''In this study, the effect of intermittent fasting (IF) on tissue-specific autophagy was examined in humans and mice.
Fasting transiently increased markers of autophagy in liver, but not skeletal muscle, in IF-mice that were fed chow or high-fat diet (HFD). However, diet-induced obese mice displayed impaired ability to alter autophagy including lysosomal function.
Fasting also did not alter markers of autophagy at the mRNA level in skeletal muscle of humans. However, reductions in markers of autophagy were noted on non-fasting days, potentially indicating a positive effect of weight loss.
Intermittent fasting activates markers of autophagy in mouse liver, but not muscle from mouse or humans''
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''Ok, the one on human is a secondary analysis of this one
https://pubmed.ncbi.nlm.nih.gov/30569640/ https://www.munideporte.com/…/documen…/ficheros/03B6A6E4.pdf which was also commented here at the time
https://www.facebook.com/groups/810890695609053/permalink/2311952662169508/
1) IF70, an IF diet at 70% of calculated baseline energy requirements per week;
2) IF100, an IF diet at 100% of calculated baseline energy requirements per week (i.e., weight maintenance);
3) DR70, a continuous restriction at 70% of calculated baseline energy requirements daily;
4) control, 100% of calculated baseline energy requirements daily
"On fed days, IF70 participants were provided with ~100% and IF100 with ~145% of energy requirements. IF groups consumed breakfast before 8 am on fasting days (~32% of energy requirements at breakfast on fasting days in IF70 and ~37% in IF100; Supporting Information Table S1), and then commenced a ~24-hour “fast” until 8 am the following day on three nonconsecutive weekdays per week. [...] All diets were matched for macronutrient composition (35% fat, 15% protein, 50% carbohydrate)."
From the secondary analysis:
"...there was no evidence of an increase in markers of autophagy in response to the 24h fast, except an increase in SQSTM1 mRNA transcript that was imposed by the IF protocol" [...] In human vastus lateralis muscle, mRNA levels of BECLIN1, SQSTM1, and LAMP2 were decreased in response to IF, but solely when assessed following a fed day.
This data suggests skeletal muscle autophagy may be dampened in women with obesity in response to weight loss, although we did not observe a correlation between the change in these markers and the degree of weight loss in this study"''
When prescribed at matched energy restriction, IF reduced weight and fat mass and improved total and low-density lipoprotein cholesterol more than DR. IF prescribed in energy balance did not improve health compared with other groups, despite modest weight loss.
pubmed.ncbi.nlm.nih.gov
